Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse Mab

概述

产品介绍
使用方法

Cat No. CHIKV-AM1005

库存：现货

Size

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产品介绍

产品名称

Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 Antibody, Mouse MAb

经验证的应用

WB (FAQProtocol), ELISA (FAQProtocol), ELISA(Det) (FAQProtocol)

相关说明

IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)

特异性

Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2
Has cross-reactivity in ELISA with
CHIKV (UVE/CHIKV/2024/RE/CNR_79903) E2 Protein (Cat# 41094-V49H)

免疫原

Recombinant Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 Protein (Catalog#40440-V08B)

制备方法

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 (Catalog#40440-V08B; ADG95913.1; Ser326-Gln666). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

来源

Monoclonal Mouse IgG2b Clone #26

纯化

Protein A

缓冲液

0.2 μm filtered solution in PBS

偶联物

Unconjugated

状态

Liquid

运输方式

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

储存条件

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CHIKV Envelope 2 mouse monoclonal antibody at 1:1000 dilution

Sample: Recombinant Protein 10 ng

Lane A: Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 Protein(Cat#41094-V49H)

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

使用方法

应用	推荐稀释比/用量
WB	1:500-1:2000
ELISA	1:1000-1:2000
ELISA(Cap)	1:1000-1:10000

备注

ELISA(Cap)：

This antibody will detect Chikungunya virus (CHIKV) (strain SL-CK1) E2 / Envelope 2 in ELISA pair set (Catalog: # SEK40440). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 40440-MM25). This antibody will detect Chikungunya virus (CHIKV) (UVE/CHIKV/2024/RE/CNR_79903) E2 / Envelope 2 ELISA Pair Set in ELISA pair set (Catalog: # SEK41094). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 40440-MM25).

请注意：最佳浓度/稀释度应由用户决定。