Recombinant Human CD19 Protein (His Tag)

概述

产品介绍
使用方法

Cat No. CD19-PH3101

库存：现货

Size

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产品介绍

纯度

For specific information on a given lot, see related technical data sheet.

生物活性

For specific information on a given lot, see related technical data sheet.

蛋白构建

Recombinant human CD19 (20-291) was expressed in CHO cells using a C-terminal His tag.

表达宿主

CHO Cells

种属

Human

分子量

Observed MW ~50-65 kDa. Calculated MW ~31 kDa.

缓冲液

Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 50-200mM imidazole, 0-0.1mM PMSF, 0.2-1.0mM DTT, 10-25% glycerol
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

Enzymes are highly recommended to be shipped at frozen temperature with dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.

稳定性 & 储存条件

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Recombinant Human CD19 Protein (His Tag): 别称

B4 Protein, Human; CVID3 Protein, Human

CD19 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.

全称

CD19 molecule

研究领域

Cancer Drug Targets

相关通路图

B Cell Receptor Signaling Pathway

参考文献

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.

使用方法

Recombinant Mouse CD19 Protein (hFc Tag), HPLC-verified: 别称

Cd19 Protein, Mouse

CD19 背景信息

全称

CD19 molecule

研究领域

Cancer Drug Targets

相关通路图

B Cell Receptor Signaling Pathway

参考文献

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.