Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated

概述

产品介绍

Cat No. CD19-PH1104

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

1.Immobilized Anti-CD19 (Research Grade Inebilizumab Biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B), the EC₅₀ is 3-9 ng/mL(QC tested).
2.Anti-CD19 (Research Grade Inebilizumab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 1.234 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
3.Anti-CD19 (Research Grade Tafasitamab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 17.630 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
4.Anti-CD19 (Research Grade Coltuximab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 5.155 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
5.Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B) captured on Biotin CAP-Series S Sensor chip, can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar) with an affinity constant of 94.700 pM as determined in an SPR assay (Biacore 8K) (QC tested).

蛋白构建

A DNA sequence encoding the Human CD19 (P15391-1) (Met1-Lys291) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Accession#

P15391-1

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Pro 20

分子量

The recombinant Human CD19 consists of 298 amino acids and predicts a molecular mass of 33.37 kDa. It migrates as an approximately 56.84 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

11880-H49H-B改.png

Immobilized Anti-CD19 (Research Grade Inebilizumab Biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B), the EC₅₀ is 3-9 ng/mL(QC tested).

Anti-CD19 (Research Grade Inebilizumab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 1.234 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

Anti-CD19 (Research Grade Tafasitamab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 17.630 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

Anti-CD19 (Research Grade Coltuximab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 5.155 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B) captured on Biotin CAP-Series S Sensor chip, can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar) with an affinity constant of 94.700 pM as determined in an SPR assay (Biacore 8K) (QC tested).

Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated: 别称

B4 Protein, Human; CVID3 Protein, Human

CD19 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.

全称

CD19 molecule

研究领域

Cancer Drug Targets

相关通路图

B Cell Receptor Signaling Pathway

参考文献

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.