Recombinant Human CD19 Protein (ECD, hFc Tag), HPLC-verified, High activity

概述

产品介绍

Cat No. CD19-PH1202

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested).

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), the EC₅₀ is 3.4-10 ng/mL(Routinely tested).
2.Loaded Biotinylated Anti-CD19 (Research Grade Inebilizumab Biosimilar) on SA Biosensor, can bind Recombinant Human CD19 Protein, hFc Tag (Cat. No. 11880-H02W) with an affinity constant of 0.575 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3.Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), Biotinylated, the EC₅₀ is 1-4 ng/mL(QC tested).

蛋白构建

A DNA sequence encoding the Human CD19 (NP_001171569.1) (Met1-Lys291) was expressed with the Fc region of human IgG1 at the C-terminus.

Accession#

NP_001171569.1

表达宿主

HEK293 Stable Cells

种属

Human

预测 N 端

Pro 20

分子量

The recombinant Human CD19 consists of 510 amino acids and predicts a molecular mass of 56.84 kDa. It migrates as an approximately 169.1 kDa band in SDS-PAGE under non-reducing conditions. The molecular weight of this protein is around 143.2 kDa verified by SEC-MALS (Routinely tested).

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), the EC₅₀ is 3.4-10 ng/mL(Routinely tested).

The purity of Recombinant Human CD19 Protein, ECD, Fc Tag, HPLC-verified (Cat. No. 11880-H02W) was more than 90% and the molecular weight of this protein is around 143.2 kDa verified by SEC-MALS (Routinely tested).

Loaded Biotinylated Anti-CD19 (Research Grade Inebilizumab Biosimilar) on SA Biosensor, can bind Recombinant Human CD19 Protein, hFc Tag (Cat. No. 11880-H02W) with an affinity constant of 0.575 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), Biotinylated, the EC₅₀ is 1-4 ng/mL(QC tested).

Recombinant Human CD19 Protein (ECD, hFc Tag), HPLC-verified, High activity: 别称

B4 Protein, Human; CVID3 Protein, Human

CD19 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.

全称

CD19 molecule

研究领域

Cancer Drug Targets

相关通路图

B Cell Receptor Signaling Pathway

参考文献

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.