Recombinant Human CD20 Protein (TrxA Tag), Amine-Labeled Biotinylated

概述

产品介绍

Cat No. CD20-PH2301

库存：现货

Size

点击咨询在线客服

产品介绍

纯度

> 80 % as determined by SDS-PAGE.

内毒素

Please contact us for more information.

生物活性

1.Loaded Obinutuzumab derived anti-Human CD20 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human CD20 Protein, TrxA Tag, Biotinylated (Cat. No. 11007-H34E-B) with an affinity constant of 14.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2.5e5 of anti-CD20 (Rituximab derived) CAR-293 cells were incubated with biotinylated Human CD20 Protein (TrxA Tag) (Cat. No. 11007-H34E-B) , and then stained with PE-conjugated Streptavidin. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (Routinely tested).
3.5e5 of anti-CD20 (Obinutuzumab derived) CAR-293 cells were incubated with biotinylated Human CD20 Protein (TrxA Tag) (Cat. No. 11007-H34E-B) , and then stained with PE-conjugated Streptavidin. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (Routinely tested).

蛋白构建

A DNA sequence encoding the human MS4A1 (NP_068769.2) (Ile141-Ser188) was expressed with a TrxA tag at the N-terminus. The purified protein was biotinylated in vitro.

Accession#

NP_068769.2

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human MS4A1 consists of 218 amino acids and predicts a molecular mass of 23.9 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

11007-H34E-B_看图王.jpg

Loaded Obinutuzumab derived anti-Human CD20 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human CD20 Protein, TrxA Tag, Biotinylated (Cat. No. 11007-H34E-B) with an affinity constant of 14.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Recombinant Human CD20 Protein (TrxA Tag), Amine-Labeled Biotinylated: 别称

B1 Protein, Human; Bp35 Protein, Human; CD20 Protein, Human; CVID5 Protein, Human; FMC7 Protein, Human; LEU-16 Protein, Human; S7 Protein, Human

CD20 背景信息

CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed in all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells are usually in the normal range but can be very low.

全称

membrane-spanning 4-domains, subfamily A, member 1

研究领域

Cancer Drug Targets

参考文献

Tedder TF, et al. (1988) Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes. Proc Natl Acad Sci. 85(1): 208-12.
Cragg MS, et al. (2005) The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 8: 140-74..
Polyak MJ, et al. (2003) A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 17(7): 1384-9.