Recombinant Human CD20 Protein (Nanodisc, Full-length, Strep & His)

概述

产品介绍

Cat No. CD20-PH1101

库存：现货

Size

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产品介绍

纯度

≥ 75 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Immobilized Obinutuzumab derived Anti-CD20 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20-Nanodisc (Full Length) Protein (Cat: 11007-H93H-NB), the EC₅₀ is 1-5 ng/mL.
2.Immobilized Rituximab derived Anti-CD20 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20-Nanodisc (Full Length) Protein (Cat: 11007-H93H-NB), the EC₅₀ is 2-8 ng/mL.
3.Immobilized Recombinant Human CD20 Protein (Nanodisc, Full-length, Strep & His Tag) (Cat: 11007-H93H-NB) at 5 μg/mL (100 μL/well) can bind Obinutuzumab derived Anti-CD20 antibody, Human IgG1, the EC₅₀ is 4.0-12.0 ng/mL (Routinely tested).
4.Loaded Obinutuzumab on proA Biosensor, can bind Recombinant Human CD20 Protein, Nanodisc, Full-length, Strep & His Tag (Cat. No. 11007-H93H-NB) with an affinity constant of 11.000 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
5.Loaded Rituximab on proA Biosensor, can bind Recombinant Human CD20 Protein, Nanodisc, Full-length, Strep & His Tag (Cat. No. 11007-H93H-NB) with an affinity constant of 14.800 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白构建

A DNA sequence encoding the Human MS4A1 (P11836) (Met1-Pro297) was expressed with a Strep tag at the N-terminus, a polyhistidine tag at the C-terminus. This protein was prepared using our Nanodisc platform. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.

表达宿主

HEK293 Cells

种属

Human

分子量

The recombinant Human MS4A1 consists of 333 amino acids and predicts a molecular mass of 36.71 kDa. It migrates as an approximately 41 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Supplied as sterile 50 mM HEPES, 150 mM NaCl, pH 7.5.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

It is supplied and shipped as liquid with dry ice.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Immobilized Obinutuzumab derived Anti-CD20 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20-Nanodisc (Full Length) Protein (Cat: 11007-H93H-NB), the EC₅₀ is 1-5 ng/mL.

Immobilized Rituximab derived Anti-CD20 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20-Nanodisc (Full Length) Protein (Cat: 11007-H93H-NB), the EC₅₀ is 2-8 ng/mL.

Immobilized Recombinant Human CD20 Protein (Nanodisc, Full-length, Strep & His Tag) (Cat: 11007-H93H-NB) at 5 μg/mL (100 μL/well) can bind Obinutuzumab derived Anti-CD20 antibody, Human IgG1, the EC₅₀ is 4.0-12.0 ng/mL (Routinely tested).

Loaded Obinutuzumab on proA Biosensor, can bind Recombinant Human CD20 Protein, Nanodisc, Full-length, Strep & His Tag (Cat. No. 11007-H93H-NB) with an affinity constant of 11.000 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Loaded Rituximab on proA Biosensor, can bind Recombinant Human CD20 Protein, Nanodisc, Full-length, Strep & His Tag (Cat. No. 11007-H93H-NB) with an affinity constant of 14.800 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Recombinant Human CD20 Protein (Nanodisc, Full-length, Strep & His): 别称

B1 Protein, Human; Bp35 Protein, Human; CD20 Protein, Human; CVID5 Protein, Human; FMC7 Protein, Human; LEU-16 Protein, Human; S7 Protein, Human

CD20 背景信息

CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed in all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells are usually in the normal range but can be very low.

全称

membrane-spanning 4-domains, subfamily A, member 1

研究领域

Cancer Drug Targets

参考文献

Tedder TF, et al. (1988) Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes. Proc Natl Acad Sci. 85(1): 208-12.
Cragg MS, et al. (2005) The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 8: 140-74..
Polyak MJ, et al. (2003) A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 17(7): 1384-9.