Recombinant Human BCMA Protein (ECD, His Tag), HPLC-verified

概述

产品介绍

Cat No. BCMA-PH1101

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Immobilized Human BAFF (Cat:10056-H01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC₅₀ of Human BCMA (Cat:10620-H08H) is 30-80 ng/mL.
2.Immobilized Mouse TNFSF13(Cat:50965-M01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC₅₀ of Human BCMA (Cat:10620-H08H) is 0.6-2.1 ng/mL.
3.293 cells were transduced with anti-BCMA-scFv and subjected to flow cytometric analysis using recombinant human BCMA (left, Cat. No. 10620-H08H, red) and a negative control protein (blue). The cells were then stained with a PE-conjugated anti-His antibody. Non-transduced 293 cells were used as a control (right).
4.Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
5.Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC₅₀ is 3-10 ng/mL (Routinely tested).
6.Anti-BCMA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 22.3 nM as determined in an SPR assay (Biacore 8K) (Routinely tested).
7.Loaded Biotinylated Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag) (Cat. No. 10056-H56H1-B) on SA Biosensor, can bind Recombinant Human BCMA Protein (ECD, His Tag) (Cat. No. 10620-H08H ) with an affinity constant of 0.853 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白构建

A DNA sequence encoding the human TNFRSF17 (NP_001183.2) (Met1-Ala54) was expressed with a polyhistidine tag at the C-terminus.

Accession#

NP_001183.2

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Met 1

分子量

The recombinant human TNFRSF17 consists of 65 amino acids and predicts a molecular mass of 7.3 kDa. As a result of glycosylation, It migrates as an approximately 16.9 and 12.4 KDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Loaded Biotinylated Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag) (Cat. No. 10056-H56H1-B) on SA Biosensor, can bind Recombinant Human BCMA Protein (ECD, His Tag) (Cat. No. 10620-H08H ) with an affinity constant of 0.853 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Immobilized Human BAFF (Cat:10056-H01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC₅₀ is 30-80 ng/mL.

Immobilized Mouse TNFSF13(Cat:50965-M01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC₅₀ is 0.6-2.1 ng/mL.

Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC₅₀ is 3-10 ng/mL (Routinely tested).

Lot-to-Lot Consistency

Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Anti-BCMA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 22.3 nM as determined in an SPR assay (Biacore 8K) (Routinely tested).

293 cells were transduced with anti-BCMA-scFv and subjected to flow cytometric analysis using recombinant human BCMA (left, Cat. No. 10620-H08H, red) and a negative control protein (blue). The cells were then stained with a PE-conjugated anti-His antibody. Non-transduced 293 cells were used as a control (right).

Recombinant Human BCMA Protein (ECD, His Tag), HPLC-verified: 别称

BCM Protein, Human; BCMA Protein, Human; CD269 Protein, Human; TNFRSF13A Protein, Human

BCMA 背景信息

Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.

全称

tumor necrosis factor receptor superfamily member 17

参考文献

Novak AJ, et al. (2004) Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival. Blood. 103 (2): 689–94.
O'Connor BP, et al. (2004) BCMA is essential for the survival of long-lived bone marrow plasma cells. J Exp Med. 199(1): 91-8.
Moser K, et al. (2006) Stromal niches, plasma cell differentiation and survival. Curr Opin Immunol. 18(3): 265-70.