Recombinant Cynomolgus, Rhesus BCMA Protein (mFc Tag), HPLC-verified

概述

产品介绍

Cat No. BCMA-PC1202

库存：现货

Size

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产品介绍

纯度

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Immobilized Recombinant Cynomolgus / Rhesus BCMA Protein (mFc Tag) (Cat: 90103-C38H ) at 5 μg/mL (100 μL/well) can bind Recombinant Human BLyS / TNFSF13B Protein (Fc & AVI Tag), Biotinylated (Cat: 10056-H42H-B), the EC₅₀ is 1.5-6.0 ng/mL (QC tested).

蛋白构建

A DNA sequence encoding the Cynomolgus, Rhesus TNFRSF17 (XP_001106892.1) (Met1-Ala53) was expressed with the Fc region of mouse IgG2a at the C-terminus.

表达宿主

HEK293 Cells

种属

Cynomolgus, Rhesus

预测 N 端

Met 1

分子量

The recombinant Cynomolgus, Rhesus TNFRSF17 comprises 286 amino acids and has a predicted molecular mass of 32.38 kDa. It migrates as approximately 38.3 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

BCMA 背景信息

Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.

全称

tumor necrosis factor receptor superfamily member 17

参考文献

Novak AJ, et al. (2004) Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival. Blood. 103 (2): 689–94.
O'Connor BP, et al. (2004) BCMA is essential for the survival of long-lived bone marrow plasma cells. J Exp Med. 199(1): 91-8.
Moser K, et al. (2006) Stromal niches, plasma cell differentiation and survival. Curr Opin Immunol. 18(3): 265-70.